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M9470100.TXT
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1994-07-02
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Document 0100
DOCN M9470100
TI Bias of culture techniques for diagnosing mixed Mycobacterium genavense
and Mycobacterium avium infection in AIDS.
DT 9409
AU Kirschner P; Vogel U; Hein R; Bottger EC; Institut fur Medizinische
Mikrobiologie, Medizinische Hochschule; Hannover, Germany.
SO J Clin Microbiol. 1994 Mar;32(3):828-31. Unique Identifier : AIDSLINE
MED/94253357
AB Disseminated Mycobacterium avium infection is a common complication in
late-stage AIDS. We describe a patient seropositive for human
immunodeficiency virus with a disseminated infection caused by
mycobacteria. Acid-fast rods were visible by microscopy in stool and
bone marrow and in specimens from skin, liver, spleen, lung, and lymph
nodes. Using molecular biology techniques and standard culture
techniques, we found evidence of a mixed mycobacterial infection with M.
genavense and M. avium. We suggest that the prevalence of M. genavense
infection in patients with AIDS is underestimated because of the bias
toward M. avium when using standard techniques for the detection and
identification of mycobacteria.
DE Adult AIDS-Related Opportunistic Infections/COMPLICATIONS/*DIAGNOSIS
*Bacteriological Techniques/STATISTICS & NUMER DATA Base Sequence Bias
(Epidemiology) Case Report Comparative Study DNA Primers/GENETICS
DNA, Bacterial/GENETICS DNA, Ribosomal/GENETICS Human HIV-1 Male
Molecular Sequence Data Mycobacterium/GENETICS/ISOLATION & PURIF
Mycobacterium avium Complex/GENETICS/ISOLATION & PURIF Mycobacterium
avium-intracellulare Infection/COMPLICATIONS/ *DIAGNOSIS Mycobacterium
Infections/COMPLICATIONS/*DIAGNOSIS Polymerase Chain Reaction Support,
Non-U.S. Gov't JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).